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. 2021 Dec 20;40:397. doi: 10.1186/s13046-021-02210-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — NEK2 is highly expressed in TNBC. A-D Analysis of TGCA data for NEK2 expression using the visual interface of the psichomics R package. A Density plots showing distribution of NEK2 gene expression in normal breast tissues, triple-negative (TNBC) and other breast cancer subtypes (Other BC) (indicated p-value estimated by Kruskal-Wallis rank sum test). B-D Density plots showing distribution of NEK2 gene expression in hormone positive (ER⁺, PR⁺) or negative (ER⁻, PR⁻) and HER2 amplified (HER2⁺) or HER2 negative (HER2^-) breast cancers (indicated p-value estimated by Wilcoxon rank sum test with continuity correction). E Representative Western blot analysis of NEK2 expression in indicated hormone-positive (ER⁺PR⁺), HER2-positive (HER2⁺) or triple-negative (TN) breast cancer cell lines. HSP90 was evaluated as loading control. F Line graph showing growth rate of MCF-7 and MDA-MB-231 cells transfected with either control (si-CTRL) or NEK2 targeting si-RNAs (si-NEK2). Number of cells at indicated time points was evaluated by IncuCyte® SX5 Live-Cell Analysis Imaging System and normalized to t0 (mean ± SD, n = 4, **p ≤ 0.01, ****p ≤ 0.0001, two-way Anova)