Table 1.
Performance of commercial reagents and platforms to detect hIL-33
| Assay | Platform | Assay criteria | ||||
|---|---|---|---|---|---|---|
| LLOQ in buffer (pg/mL) | LLOQ in VH (pg/mL) | sST2 tolerance (ng/mL) | Immunodepletion (%) | Sample volume (µL) | ||
| R&D Human IL-33 Quantikine Kit | ELISA | 6.25 | 62.5 | 1 | n/a | 50 |
| Biolegend LEGEND MAX™ Human IL-33 Kit | 15.6 | n/a | 10 | n/a | 50 | |
|
Invitrogen IL-33 Human Kit |
1.6 | 3.2 | 1 | 81 | 50 | |
| Rb xhIL-33 (Pepro)/Biotin-goat xhIL-33 (R&D) | 0.8 | 8 | 0.25 | n/a | 25 | |
| Mu xhIL-33 (Enzo)/Biotin-mu xhIL-33 (Novus) | 20 | n/a | 0.5 | 59 | 25 | |
|
U-PLEX Human IL-33 MSD Kit |
ECL | 0.6 | 2.4 | 0.5 | 60 | 25 |
| Bio-Plex Pro™ Human Th17 Cytokine 15-Plex Kit | Luminex | 1.3 | 2.6 | 0.016 | n/a | 50 |
| Mu xhL-33 (Enzo)/Biotin-mu xhIL-33 (Novus) | Quanterix | 20 | n/a | n/a | n/a | 150 |
Parameters that did not meet our assay criteria selection are highlighted in bold; n/a represents parameters not tested