Table 2.

Standard curves performance of reduced and total hIL-33 ELISA

	Nominal conc (pg/mL)	Measured mean conc (pg/mL)	% Recovery	% CV
Reduced hIL-33 ELISA	1000	991.5	99.2	1.6
	400	408.2	102.1	2.8
	160	158.5	99.0	1.7
	64	63.6	99.4	1.5
	25.6	26.2	102.3	7.0
	10.2	11.0	107.6	5.6
	4.10	3.83	93.6	9.1
Total hIL-33 ELISA	1000	1007.4	100.7	0.7
	400	407.5	101.9	2.5
	160	159.6	99.7	2.4
	64	64.7	101.1	8.4
	25.6	27.6	107.9	8.4
	10.2	9.56	93.4	9.6
	4.10	4.54	110.8	10.5

n = 6 independent assay runs. LLOQ = 4.1 pg/mL, ULOQ = 1000 pg/mL