Table 6.

Accuracy test as measured by spike recovery of reduced hIL-33 in matrices using reduced hIL-33 iPCR assay

hIL-33 (pg/mL)	VH (1:2)	AH (1:2)	Serum (1:4)	Plasma (1:2)	NF (1:2)
Endogenous conc	< 0.078	0.59	2.24	5.73	7.91
Spiked conc. (low)	0.47	0.47	0.45	0.36	0.45
Measured conc	0.41	1.14	3.14	7.26	9.78
Expected conc. (endo + spike)	0.47	1.06	2.69	6.09	8.36
% Recovery	86.5	107.1	117.0	119.2	117.0
Endogenous conc	< 0.078	0.59	2.24	4.78	7.91
Spiked conc. (mid)	1.11	1.36	1.51	1.40	1.51
Measured conc	0.93	1.73	3.48	5.90	11.0
Expected conc. (endo + spike)	1.11	1.95	3.75	6.18	9.42
% Recovery	84.1	88.7	92.8	95.5	116.8
Endogenous conc	< 0.078	3.21	2.24	5.73	7.91
Spiked conc. (high)	4.25	4.25	4.48	5.22	4.48
Measured conc	3.49	6.9	5.44	10.48	14.7
Expected conc. (endo + spike)	4.25	7.47	6.72	10.95	12.39
% Recovery	82.1	92.4	81.0	95.8	118.3