FIG. 6.

The GIP complex eluted from outer membrane vesicles after BN-PAGE is functional. (A) Schematic diagram showing isolation of the 400K GIP complex. (B and C) Current traces from a bilayer fused with 400K complex containing liposomes under symmetric conditions (250 mM KCl, 10 mM MOPS-Tris [pH 7.0] on both sides of the membrane). The bottom trace of panel C shows a time scale-expanded current recording with high time resolution. (D) Current-voltage relationship of the two most frequent conductance states with the same symmetric KCl concentrations as in panel B. (E) Current-voltage relationship of the main conductance level at asymmetric buffers (cis, 250 mM KCl; trans, 20 mM KCl) for determination of reverse potential.