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. Author manuscript; available in PMC: 2021 Dec 20.
Published in final edited form as: Science. 2021 Oct 8;374(6564):eabd4453. doi: 10.1126/science.abd4453

Fig. 2. The IVC reveals a defect in DNA binding by the RpaA-R121Q mutant.

Fig. 2.

(A) Rhythms of bioluminescence generated by PkaiBC::luc expression were monitored for entrained WT (black), ΔkaiC (blue), ΔrpaA (gray), and rpaA-R121Q (salmon) cyanobacterial strains. Each plot represents an average of six biological replicates. kcps, kilocounts per second. (B) Rhythms of RpaA phosphorylation or (C) FA of PkaiBC DNA in IVC reactions containing either SasA or CikA, as in Fig. 1, A and B.