Figure 7.

PRV infection induced the expression of GADD34 in vitro and in vivo. PK15 cells were infected with PRV at an A MOI of 0.1 or B MOI of 1.0 at 0, 6, 9, 12, and 24 hpi. Then, the protein level of GADD34 was determined by Western blot analysis; the intensities of the GADD34 bands were quantified by densitometry and normalized to β-actin and are shown as fold changes (right panel). The values are presented as the mean ± SD of triplicate experiments. ** p < 0.01; *** p < 0.001. C BALB/c mice were intraperitoneally inoculated with PRV (1 × 10⁶ PFU) and were then euthanized at 0, 24, 48, and 72 hpi. The protein level of GADD34 in the lungs was determined by Western blot analysis; the intensities of the GADD34 bands were quantified by densitometry and normalized to β-actin and are shown as fold changes (right panel). The values are presented as the mean ± SD of triplicate experiments. * p < 0.05.