TABLE 3.
SSR phenotypes of wild-type and nuclease-deficient cells
| Genotype | H−/L− ratioa |
|---|---|
| Wild-type diploid | 0.03 ± 0.01 |
| din7::KANMX/din7::KANMX | 0.03 ± 0.01 |
| exo1::hisG/exo1::hisG | 0.048 ± 0.01 |
| rad27::leu2::hisG/rad27::leu2::hisG | 0.22 ± 0.04 |
a
Diploid strains ABX367, ABX382, ABX451, and ABX456 were transformed with the shortest DNA fragment, which contains 91 and 36 bp of DNA flanking the URA3 gene. The ratio of transformants that were due to fragment insertion into the HIS3 allele (His− Leu+ Ura+) versus those that were due to insertion into the his3::ura3::LEU2 allele (His+ Leu− Ura+) was determined as described in the text. The means from a minimum of five trials ± two standard errors are reported. Means that differ by greater than two standard errors are different at the 95% confidence level.