TABLE 4.
Published proteomic analyses in Brassica species during interaction with black rot.
Species | Plant organ | Time point (Tissue collection) | Methodology | Objective | Inference | References |
Brassica oleracea var. capitata | Leaves | 24-h after inoculation (hai) |
2-DE | To identify the Brassica oleracea proteins during early infection by Xcc | Peroxiredoxin precursor protein decreased in the susceptible genotype inoculated with Xcc. Proteins involved in photosynthesis were also modulated by Xcc infection. | Costa et al., 2014 |
Brassica oleracea | Leaves |
5-, 10-, and 15- days after inoculation (DAI) | 2-DE, MALDI-TOF | To identify the proteins in susceptible and resistant Brassica oleracea in response to Xcc infection | Susceptible interaction showed a clear reduction in the abundance of proteins involved in energy metabolism and defence whereas in the resistance interaction, these proteins showed an opposite behavior. Resistance was correlated with the ability of the plants to keep sufficient photosynthesis metabolism activity to provide energy supplies necessary for an active defence. | Villeth et al., 2016 |
Brassica oleracea | Leaves | 24-h after Xcc inoculation (hai) | 2-DE, MALDI TOF-TOF | Identification of Brassica oleracea resistance-related proteins at an early stage of infection by Xcc | Reduction of photosynthesis-related proteins was observed both in the resistance and susceptible interactions. Also, decreased abundance of ubiquitin (to stop the bacteria from using ubiquitination pathway) and malate dehydrogenase (to reduce energy metabolism in the early stage of infection) were found playing important role in the resistance mechanism against Xcc. | Ribeiro et al., 2018 |
Brassica oleracea | Leaves | 3- and 12-days post-infection | MALDI-TOF-TOF | To investigate the molecular changes at the protein level in Brassica oleracea plants infected by Xcc | Proteins shared between early and late response were related to photorespiration, calvin cycle and light reactions, and are strongly down-regulated after Xcc infection. Proteins related with glucosinolates degradation (myrosinase) were up-regulated in both early and late response. Proteins related with signalling were up-regulated in late response | Tortosa et al., 2018a |
Brassica oleracea var. capitata | Leaves | 24-h after infiltration (hai) |
LC-MS/MS | Proteomic analysis of cabbage inoculated with Xcc and functional validation of Brassica oleracea endochitinase involved in resistance to Xcc | Differentially abundant proteins were involved in cell metabolism, protein biosynthesis, processing and degradation, photosynthesis and disease/defence response. A CHI-B4 like gene, encoding an endochitinase showed a high increased abundance in resistant Xcc-inoculated leaves and was functionally validated in Arabidopsis thaliana | Santos C. et al., 2019 |
Brassica napus | Leaves | 14-days after inoculation | LC–MS/MS | Quantitative proteomic analysis of susceptible and resistant Brassica napus cultivars infected with Xcc | All proteins involved in protein degradation and C2 oxidative cycle and glycolysis, innate immunity-related proteins (zinc finger domain (ZFD)-containing protein, glycine-rich RNA-binding protein (GRP) and mitochondrial outer membrane porin), PS I proteins, ATP synthase, and ferredoxin-NADP+ reductase, redox-related proteins were up-regulated in the resistant cultivar (cv. Capitol) whereas Photosystem II-related proteins were down-regulated | Islam et al., 2021 |
Brassica oleracea | Proteome of Xcc extracted from leaves of infected B. oleracea | 0-, 1-, 2-, 4- and 6-days after inoculation (DAI) |
2-DE, MALDI-TOF/TOF | To characterize the protein expression of Xcc in close interaction with B. oleracea | Several proteins expressed in vivo were identified and were related mainly to metabolism. | Andrade et al., 2008 |
Brassica oleracea | Proteome of Xcc extracted from leaves of infected B. oleracea | 0-, 1-, 2- and 3-days after inoculation (DAI) |
2-DE | To analyze the expressed proteins of Xcc exclusive to resistance interaction in B. oleracea |
Protein profile comparison revealed a group of proteins exclusive to the resistance interaction like Rubisco. Upregulation of proteins involved in photosynthesis in the resistance interaction included intact Rubisco subunits and an oxygen-evolving protein. The presence of different isoforms of the same protein in the resistance and the susceptible interactions indicated that the same protein may play different roles depending on the types of interaction. | Villeth et al., 2009 |
Brassica oleracea | Proteome of Xcc extracted from leaves of infected B. oleracea | 0-, 24-, 48-, 72-, and 120-h after inoculation (hai) | 2D-nano UPLC/MS | To study the interaction of B. oleracea–Xcc using an in vivo system to identify proteins involved in pathogenicity | Pathogenicity related proteins [acetylornithine (ArgD)] and several defence and stress-related proteins (lipoxygenase, annexins, apocitocrome f, antimicrobial compound phytoalexin) were observed in the susceptible (REK) and resistant (REU) Brassica plants, respectively. Also, proteins associated with photosystems were identified in the resistant plants. A model of Xcc-susceptible host interaction was proposed and showed that Xcc increases the abundance of several crucial proteins for infection and cell protection. | Santos et al., 2017 |