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Fluorescence images of 1 μm beads in 3D hydrogels measured by Gaussian light-sheet (a) and single-shot C-light-sheet microscopy (b-d). The detection objectives were 4×/0.13 for (a, b) and 50×/0.8 for (c, d). All images were maximum intensity projected. Scale bars, 10 μm (white) and 200 μm (yellow).
