FIG. 2.

Map of the region of mouse necdin analyzed for CpG methylation status. The gene structure is shown at the top, where the black box represents the open reading frame and the +1 and ATG are the transcriptional and translational start sites, respectively. CpG dinucleotides are shown as vertical bars below the map. The arrowhead points to the BamHI restriction site used to insert the lacZ gene into the Ndn knockout mouse. The relative positions of the primers used for nested PCR are shown, and the second set of primers (NEC41F-NEC48R) is in boldface. The 560-bp region analyzed covers CpG sites 1 to 39 and is underlined in the C57BL/6 type sequence. For some of the samples, other primers were used to amplify the same region (see Materials and Methods). Single-nucleotide polymorphisms between C57BL/6 and SPRET and between C57BL/6 and CAST are indicated by an asterisk and are as follows: a, CAST, CGGTC; and b, SPRET and CAST, CGCGT. A 5-bp insertion was found in SPRET at c, SPRET CGCATCGCATCG. The changes from the C57BL/6 sequence are in boldface. CpG dinucleotides are underlined.