Table 1.
LGP of CRE/ESKAPE and Other Pathogensa
| LGPb | strainc | metal complexd | protein ligandse | aa | massf | pIg | E. coli K-12 orthologueh | NCBI refi | PDB |
|---|---|---|---|---|---|---|---|---|---|
| Commensal E. coli | |||||||||
| FecA | MG1655 | FeCit | ND | 741 | 81 707 | 5.61 | NSI | NP_418711.1 | 1PNZ |
| FepA | MG1655 | FeEnt | colB, D; H8, mE495 | 724 | 79 771 | 5.4 | 53% IroN | NP_415116.1 | 1FEP |
| FhuA | MG1655 | Fc | colM, T1, T5, φ80 | 714 | 78 742 | 5.3 | NSI | NP_414692.1 | 1BY5 |
| Fiu | MG1655 | FeDHBS | ND | 727 | 78 432 | 5.75 | NSI | NP_415326.1 | 6BPN |
| FhuE | MG1655 | FeRta | ND | 693 | 77 411 | 4.89 | NSI | NP_415620.1 | 6E4V |
| Cir | MG1655 | FeDHBS | colIa, Ib, | 638 | 71 149 | 5.2 | 35% FepA | NP_416660.1 | 2HDF |
| BtuB | MG1655 | B12 | ColE1, E3, BF23 | 594 | 66 325 | 5.35 | NSI | NP_418401.1 | 1NQE |
| Pathogenic E. coli | |||||||||
| YddB | UPEC 042 | ND | ND | 771 | 87 206 | 6.06 | NSI | CBG34449.1 | 6OFR |
| IutA | 083:H1j | FeAbn | cloacin DF13, colV | 708 | 78 061 | 5.23 | NSI | WP_000973516.1 | ND |
| IroN | 083:H1j | FeGEnt | ND | 701 | 76 525 | 5.79 | 52% FepA | ADR29866.1 | ND |
| YncD | UPEC C15 | ND | ND | 677 | 74 900 | 5.32 | NSI | AKC11926.1 | 6V81 |
| FyuA | 0157:H7 | FeYbt | pesticin | 551 | 71 387 | 5.52 | NSI | EFB2704300.1 | ND |
| ChuA | 0157:H7 | Hn | ND | 632 | 69 436 | 5.27 | NSI | NP_312407.1 | ND |
| LGP1 | 0157:H7 | ND | ND | 687 | 76 150 | 5.48 | NSI | QGF16871.1 | ND |
| LGP2 | 0157:H7 | ND | ND | 634 | 71 005 | 5.69 | NSI | QGF15879.1 | ND |
| K. pneumoniae | |||||||||
| FepA4 | Kp52.145 | FeEnt | ND | 728 | 80 070 | 5.67 | 72% FepA | WP_004179434.1 | ND |
| FepA1 | Kp52.145 | FeEnt | ND | 717 | 79 665 | 5.41 | 81% FepA | CDO13414.1 | ND |
| FhuA | Kp52145 | Fc | ND | 715 | 79 054 | 5.34 | 89% FhuA | WP_048972727.1 | ND |
| IutA | hvKP1 | FeAbn | cloacin DF13 | 708 | 78 043 | 5.23 | NSI | CDO11693.1 | ND |
| Fiu | KP52145 | FeDHBS | ND | 727 | 78 023 | 5.71 | 77% Fiu | WP_171841556.1 | ND |
| FepA2 | KP52145 | ND | ND | 701 | 77 382 | 5.31 | 51% FepA | CDO16709.1 | ND |
| FhuE | KP52145 | FeRTAl | ND | 695 | 76 897 | 5.25 | 50% FhuE | AYK02175.1 | ND |
| IroN | KP52145k | FeGEnt | ND | 700 | 76 760 | 6.41 | 51% FepA | WP_042940746.1 | ND |
| FcuA | KP52145 | ND | ND | 703 | 76 166 | 5.57 | NSI | EMB11413.1 | ND |
| YncD | hvKP1 | ND | ND | 677 | 74 569 | 5.75 | NSI | EMB10697.1 | ND |
| FyuA | hvKP1 | FeYbt | pesticin | 652 | 71 400 | 5.52 | NSI | CDO15344.1 | ND |
| Cir | Kp52.145 | FeDHBS | 632 | 70 367 | 5.35 | 82% Cir | EMB11539.1 | ND | |
| ChuA | hvKP1 | Hn | ND | 613 | 67 571 | 5.27 | 100% ChuA | WP_001322816.1 | ND |
| BtuB | KP52145 | B12 | ND | 592 | 66 035 | 5.32 | 57% BtuB | CDO16333.1 | ND |
| LGP1 | KP52145 | ND | ND | 737 | 81 135 | 5.62 | NSI | QDA45483.1 | ND |
| LGP2 | KP52145 | ND | ND | 680 | 74 658 | 5.64 | 33% FhuA | EMB11926.1 | ND |
| A. baumannii | |||||||||
| Fiu | 17978 | FeDHBS | ND | 771 | 84 285 | 6.89 | 34% Fiu | AZM39353.1 | ND |
| BfnH | 17978 | FeBfn | ND | 728 | 80 491 | 5.84 | NSI | ABO12082.2 | ND |
| FepA | 17978 | FeEnt | ND | 730 | 80 248 | 5.67 | 45% FepA | WP_005135700.1 | ND |
| PiuA | 17978 | FeDHBS | ND | 736 | 80 054 | 6.23 | 31% Fiu | ABO10929.1 | 5FP1 |
| PirA | 17978 | FeDHBS | ND | 714 | 78 022 | 5.43 | NSI | SCX98474.1 | 5FR8 |
| BauA | 17978 | FeAcn | ND | 712 | 77 497 | 7.6 | NSI | ABO12804.2 | ND |
| BauA | 19606 | FeAcn | ND | 703 | 76 016 | 5.62 | NSI | WP_001073039.1 | 6H7V |
| FhuA | 19606 | Fc | ND | 679 | 75 739 | 5.59 | 25% FhuA | ABO12348.2 | ND |
| FbsN | 19606 | FeFbn | ND | 629 | 68 763 | 6.82 | 25% FhuA | ABO12983.2 | ND |
| BtuB | 19606 | B12 | ND | 598 | 65 781 | 5.71 | 25% BtuB | ABO13283.2 | ND |
| LGP1 | 19606 | ND | ND | 862 | 93 996 | 5.08 | NSI | CAA0247590.1 | ND |
| LGP2 | 19606 | ND | ND | 781 | 88 811 | 6.63 | 34% FecA | ABO13864.1 | ND |
| LGP3 | 19606 | ND | ND | 697 | 78 847 | 5,8 | NSI | ABO13728.2 | ND |
| LGP4 | 19606 | ND | ND | 674 | 77 325 | 5.52 | NSI | EEX02122.1 | ND |
| LGP5 | 19606 | ND | ND | 681 | 75757 | 5.48 | 25% FhuA | ABO11495.2 | ND |
| LGP6 | 19606 | ND | ND | 669 | 74 690 | 5.18 | 42% FepA | ABO13298.2 | ND |
| P. aeruginosa | |||||||||
| HxuA | PAO1 | Hn | ND | 965 | 95 071 | 7.33 | NSI | CRQ69633.1 | ND |
| HasR | PAO1 | Hn | ND | 855 | 94 205 | 5.85 | NSI | NP_252098.1 | ND |
| FpvA | PAO1 | FePvd | pyocins S2, S3, S4 | 772 | 86 469 | 5.27 | NSI | NP_251088.1 | 2IAH |
| PupA | PAO1 | FePch | ND | 777 | 86 005 | 5.28 | NSI | AMU01031.1 | ND |
| FoxA | PAO1 | FxB | ND | 773 | 85 273 | 5.05 | 32% FhuA | NP_251156.1 | 6196 |
| ChuA | PAO1 | Hn | ND | 737 | 81 892 | 5.99 | NSI | AAC13289.1 | ND |
| FepA1 | PAO1 | FeEnt? | ND | 735 | 80 919 | 5.85 | 71% FepA | MXH37568.1 | ND |
| PiuD | PAO1 | FeDHBS | ND | 731 | 80 149 | 5.68 | NSI | WP_132667204.1 | 5NEC |
| FepA2 | PAO1 | FeEnt? | ND | 717 | 79 687 | 5.19 | 81% FepA | MXH36562.1 | 5NEC |
| P. aeruginosa | |||||||||
| PfeA | PAO1 | FeEnt | ND | 721 | 78 503 | 5.8 | 60% IroN | NP_251378.1 | 6Q5E |
| PiuA | PAO1 | ND | ND | 729 | 78 313 | 5.7 | 77% Fiu | MXH35875.1 | 5FOK |
| ChtA | PAO1 | FeRTAl | ND | 714 | 78 166 | 5.45 | NSI | PTC33848.1 | ND |
| IutA | PAO1 | FeAbn | ND | 708 | 78 161 | 4.96 | 72% IutA | MXH36073.1 | ND |
| PirA | PAO1 | FeDHBS | ND | 714 | 77 992 | 5.43 | 56% FepA | AAG04320.1 | 5FP2 |
| FhuA | PAO1 | Fc | ND | 702 | 77 881 | 5.37 | 61% FhuA | MXH37021.1 | ND |
| IroN | PAO1 | FeGEnt | ND | 714 | 77 866 | 5.38 | 60% IroN | WP_058129121.1 | ND |
| FptA | PAO1 | FePch | pyocin E5 | 682 | 75 597 | 5.58 | NSI | NP_252911.1 | 1XKW |
| FhuE | PAO1 | FeRTAl | ND | 689 | 75 467 | 5.14 | 46% FhuE | CRQ23141.1 | ND |
| FvbA | PAO1 | FeVbn | ND | 665 | 73 731 | 5.46 | NSI | WP_003093526.1 | ND |
| Cir | PAO1 | FeDHBS | ND | 632 | 70 398 | 5.31 | 80% Cir | MXH34319.1 | ND |
| BtuB | PAO1 | B12 | ND | 598 | 66 521 | 5.38 | 57% BtuB | MXH38591.1 | ND |
| LGP1 | PAO1 | ND | ND | 821 | 92 251 | 6.39 | NSI | BAQ41081.1 | ND |
| Y. pestis | |||||||||
| HasR | KIM6+ | Hn | ND | 795 | 89 654 | 7.96 | 56% HasR | WP_002209485.1 | ND |
| IutA | KIM6+ | FeAbn | ND | 745 | 82 439 | 5.36 | 67% IutA | WP_087813403.1 | ND |
| FhuE | KIM6+ | FeRTAl | ND | 717 | 79 827 | 7.8 | 27% FhuE | WP_002211883.1 | ND |
| FhuA | KIM6+ | Fc | ND | 716 | 78 382 | 5.99 | 26% FhuA | AAS63640.1 | ND |
| huA | KIM6+ | Hn | ND | 690 | 75 802 | 5.24 | 68% ChuA | WP_002209062.1 | ND |
| Cir | KIM6+ | FeDHBS | ND | 679 | 75 555 | 6.0 | 38% Cir | WP_071526008.1 | ND |
| BtuB | KIM6+ | B12 | ND | 672 | 72 172 | 5.45 | 65% BtuB | WP_058987704.1 | ND |
| Psn | KIM6+ | FeYbt | pesticin | 651 | 71 442 | 5.62 | NSI | AAC69592.1 | 4EPA |
| LGP1 | KIM6+ | Cu++? | ND | 698 | 76 423 | 8.82 | 28% BtuB | WP_002208882.1 | ND |
| LGP2 | KIM6+ | Hn? | ND | 667 | 76 274 | 5.34 | 45% YoeA | WP_002211632.1 | ND |
| LGP3 | KIM6+ | FeDHBS | ND | 678 | 74 129 | 6.2 | 31% Cir | AAM84435.1 | ND |
| Other Gram (−) Pathogens | |||||||||
| HasR | S. marscescens m | Hn | ND | 865 | 94 847 | 6.2 | NSI | CAE46936.1 | 3CSN |
| FauA | B. pertussis | alcaligin | ND | 699 | 77 593 | 6.75 | NSI | WP_014905926.1 | 3EFM |
| FrpB | N. meningitidis n | FeEnt? | ND | 692 | 76 823 | 9.42 | NSI | AAF42315.1 | 4AIP |
a
We identified 79 LGP that participate in the uptake of ferric siderophores, heme or other metal complexes. This list, that is not fully comprehensive, illustrates the breadth of metal chelate recognition in Gram (−) bacterial pathogens. We used sequences of the mature proteins for analysis by CLUSTALΩ, the results of which appear in Figures S1 and 5.
b
LGP reflect standard nomenclature; if function is unknown, the protein is enumerated: e.g., EcoLGP1, EcoLGP2, etc.
c
Bacterial strain from which the genomic information originated.
d
Ferric siderophore or metal porphyrin; see text for abbreviations.
e
Abbreviations: col, colicin; m, microcin.
f
Mass (Da) of the mature protein.
g
Isoelectric point of the mature protein.
h
Extent of identity to the closest homologue in E. coli K-12 strain MG1655; NSI; NSI, no significant identity (i.e., <25%).
i
Entries originated from the NCBI PROTEIN database.
j
Structural gene resides on pNRG857c in strain O83:H1.
k
Structural gene resides on pLVPK in strain Kp52.145, also called pII.
l
LGP that recognize RTA often also bind FxB or coprogen.
m
S. marcescens strain SM365.
n
N. meningitidis strain MC58.