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. 2001 Apr;21(7):2413–2422. doi: 10.1128/MCB.21.7.2413-2422.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 8 — Tests of EKLF interaction with CBP in vivo. Wild-type EKLF (lane 1) or K288R (lane 2), K302R (lane 3), or K288R/K302R (lane 4) mutant EKLF was transfected into COS7 cells, and whole-cell extracts (400 μl) were subjected to immunoprecipitation with anti-CBP antibodies. Immunoprecipitated proteins were resolved, blotted, and probed with 4B9 anti-EKLF antibody. Analysis of the input lysates (20 μl) used for the immunoprecipitation is on the top, and that of the pellet is on the bottom. The location of EKLF is shown; the asterisk indicates nonspecific (immunglobulin heavy chain) signals from the immunoprecipitating antibodies.