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. 2021 Dec 6;8:732042. doi: 10.3389/fmed.2021.732042

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Copyright © 2021 Hu, Wang, Wan, Ma, Zhao and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tangshen Formula inhibited myocardial fibrosis in KKAy mice. (A) Immunohistochemistry (IHC) of collagen III (ColIII, original magnification, 200×), collagen I (ColI, original magnification, 200×), and alpha smooth muscle actin (α-SMA, original magnification, 200×). (B) IHC and semi-quantitative analyses for collagens I and III, and α-SMA. Data are expressed as mean ± SEM (n = 6). *p < 0.05, **p < 0.01 TSF-treated group vs. KKAy group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 KKAy group vs. C57BL/6J group. (C) Western blot and semi-quantitative analyses of ColI, ColIII, and α-SMA expressions.