FIGURE 2.

CIRP interacts with CD63, a member of the tetraspanin family intracellularly. (A) Molecular binding between CIRP and CD63 was predicted by 3D computational modeling. (B) RAW 264.7 cells challenged with LPS for 20 h and peritoneal cells isolated from mice (C) 4 h after LPS injection or (D) 20 h after CLP were lysed and immunoprecipitated with anti-CD63 Ab or IgG control. Precipitated proteins were subjected to WB of anti-CIRP Ab. CD63 levels of the total lysates were assessed by WB for the internal control. The experiment was repeated three times.