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. 2021 Dec 20;16(12):e0247261. doi: 10.1371/journal.pone.0247261

Fig 7. The excised Poldip2 allele is null and does not affect Tmem199 expression.

Fig 7

Gene expression was measured in liver samples from mice with indicated genotypes. Bar graphs represent m ± SEM (n = 4 mice in each group). A: Poldip2 (left) and Tmem199 (right) mRNA expression was measured by qPCR. *** P<0.001 and ns P>0.05, vs. WT. B: POLDIP2 and β-TUBULIN protein expression was assessed by Western blotting (top, each lane represents a different mouse) and quantified by densitometry (bottom). * P<0.05 and *** P< 0.001, vs. WT. The absence of POLDIP2 protein expression in Hom mice indicates that they are complete knockouts.