| Southern internal probe |
forward |
TGGAGGTGCGTTGAGCAGAAGC
|
340 bp |
| reverse |
TCTTTAGCTACTACCCAGTCCTGAGGCC
|
| Southern 3’ external probe |
forward |
GGAACCCAATGTTTCTTATTGCTAAATTAAGG
|
519 bp |
| reverse |
CCAGCTCTCCTGTTCTAACTGGCAGTTT
|
| Screening ES cells |
p1 |
GGAACTTCCTGACTAGGGGAGGAGTAGAAG
|
3.3 kb |
| p2 |
GCAGGGTAGTGTAGGCAGGGAACATAA
|
| Long 5’ PCR |
p3 |
CAAGTAACGCTTCTTAGGCGAATCC
|
5.2 kb |
| p4 |
CCATGATCCCTCCCAGCAAATGACA
|
| Long 3’ PCR |
p5 |
TCTTGGTTTCTTGATGCACAGT
|
4.2 kb |
| p6 |
GGCTCAGACTCACAACAATAATCATGC
|
| Floxed Poldip2 genotyping around 5’ loxP |
p7 |
GCACAGTTGGACATCTTTCTGTTAATCTCAC
|
516 (WT) & 601 bp |
| p8 |
GCCCAGAGCTGACCTAACCTC
|
| Floxed Poldip2 genotyping around 3’ loxP |
p9 |
CCGTGACTGCCCACATATAGTAAGTAAGC
|
710 (WT) & 808 bp |
| p10 |
GCTGTCATCATGGTTAGCCAAGAAGG
|
| Amplification of duplication junction for sequencing |
p11 |
CACCCTAGACACCAACACTAACATCT
|
1,044 bp |
| p12 |
GGATCTCTCGCTCATGCACAACG
|
| Genotyping of duplication junction |
p13 |
CAGGCAATCTGACTTCTTAGCACAC
|
416 bp |
| p12 |
see above |
| Excised Poldip2 genotyping |
p14 |
CTTCTTGGTTTCTTGATGCACAGT
|
404 (WT) & 281 bp |
| p15 |
GTTTTGCCTTTCACCTCCTTAGAGCC
|
| p16 |
ACATTATACGAAGTTATGTACGCCTA
|
|
Selected primers for duplication mapping
|
| Lepr (negative control) |
forward |
GGGTTAGAGATCTTTCATCTTTAGC
|
326 bp |
| reverse |
ATGCCCTGAAAATCAAGCATA
|
| Chr11 @ -28 kb |
forward |
TACACCAGCCCCAGAAGGATCG
|
297 bp |
| reverse |
GATGTATCTCTTTCACCTGCATGGTT
|
| Chr11 @ -171 kb |
forward |
GACATTGCTAAAGACCCCGATGC
|
144 bp |
| p12 |
see above |
| Chr11 @ -172 kb |
forward |
GTCAGGTTCTTGATAAGCTCGACT
|
171 bp |
| reverse |
CTGGTACTGTTTAGCTCTACAACGC
|
| Chr11 @ 52 kb |
forward |
GCAGAGCCAGGCATTACCTT
|
114 bp |
| reverse |
GCTTTGTCTAGGGCTTAGCTTACCA
|
| Chr11 @ 133 kb |
p11 |
see above |
116 bp |
| reverse |
TCTGTTTTCCACCAGGGACAATGC
|
| Chr11 @ 134 kb |
forward |
ATCAAATAAAACCACTGAACATTCATCCGA
|
75 bp |
| reverse |
TTTTGCCTGAAGCAGTACGTCA
|
|
Primers for RT-qPCR with LightCycler instrument
|
| Poldip2 |
forward |
AGTTCGAGGACGAGGGGTGGT
|
171 bp |
| reverse |
GAATGGTGGGATCCGGACATCAAAG
|
| Tmem199 |
forward |
CAATGTCACTTGTCAGGATG
|
168 bp |
| reverse |
GCCATTTCTGTGAAGACATAC
|
| Tnfaip1 |
forward |
AAATAGAGGCTTTGGCTTTC
|
143 bp |
| reverse |
AAGAGACAAACTGGAAACAC
|
| Nox4 |
forward |
CTGGTCTGACGGGTGTCTGCATGGTG
|
413 bp |
| reverse |
CTCCGCACAATAAAGGCACAAAGGTCCAG
|
| Peptidylprolyl isomerase A (PPIA) |
forward |
AGCTCTGAGCACTGGAGAGA
|
178 bp |
| reverse |
GCCAGGACCTGTATGCTTTA
|
| Beta-2 microglobulin (B2M) |
forward |
GGCCTGTATGCTATCCAGAA
|
198 bp |
| reverse |
GAAAGACCAGTCCTTGCTGA
|
| Hypoxanthine phosphoribosyl transferase 1 (HPRT) |
forward |
GCTGACCTGCTGGATTACAT
|
242 bp |
| reverse |
TTGGGGCTGTACTGCTTAAC
|
|
Primers for RT-qPCR with QuantStudio7 instrument
|
| Poldip2 |
forward |
GAGACCACCGAGAACATCCG
|
72 bp |
| reverse |
GTGGGAATTCTGGGCTTCCCTCA
|
| Tmem199 |
both |
see above |
168 bp |
| B2M |
both |
see above |
198 bp |
| HPRT |
forward |
see above |
279 bp |
| reverse |
GGTCCTTTTCACCAGCAAGCT
|
| PPIA |
both |
see above |
178 bp |
| Ribosomal protein L13a |
forward |
ATGACAAGAAAAAGCGGATG
|
215 bp |
| reverse |
CTTTTCTGCCTGTTTCCGTA
|
| Phosphoglycerate kinase 1 |
forward |
GCAGATTGTTTGGAATGGTC
|
185 bp |
| reverse |
TGCTCACATGGCTGACTTTA
|
| Mitochondrial Cytochrome C oxidase I |
forward |
TGTCTATTGGCTTTCTAGGC
|
240 bp |
| reverse |
AAACAATTCCGGTTAGACCA
|
| Mitochondrial Cytochrome C oxidase III |
forward |
TACACTATTAACCCTTGGCCTA
|
223 bp |
| reverse |
GCCTCCTAGATCATGTGTTG
|
| Mitochondrial NADH dehydrogenase 1 |
forward |
CATTCTAATCGCCATAGCC
|
130 bp |
| reverse |
TAATTTTATGGCGTCTGCAA
|
| Collagen IV α1 |
forward |
GCAGAGATGGTCTTGAAGGA
|
133 bp |
| reverse |
AAACCTGGGTCTCCTTTGTC
|
| Collagen IV α2 |
forward |
GCATGAACAAGCTCTGGAGT
|
186 bp |
| reverse |
TGGAGAGCCAGTAGGACTTG
|
| Collagen IV α6 |
forward |
TAACCAGGATCTGGGATTTG
|
181 bp |
| reverse |
GATATACTGGGGAATCTGGG
|
| Collagen XVIII α1 |
forward |
GTAGATTCTATAGGAGCTGAGAC
|
118 bp |
| reverse |
CTCCCTTTTGTCCTTTCATAC
|
| Integrin α3 |
forward |
TACAACTGGAAAGGAAACAG
|
111 bp |
| reverse |
CACCGTGTACCCAATATAAAG
|
| Integrin α5 |
forward |
GATCCTTTGGTGTGGACAAG
|
207 bp |
| reverse |
AGTTCCACCTCGAAGCCTAT
|
| Filamin α |
forward |
GGAGACGGTGATTACTGTGG
|
222 bp |
| reverse |
GCCAAAGCTGTAACTTGGAA
|
| Tensin 1 |
forward |
CCCCACAGATGAATCAAAAG
|
208 bp |
| reverse |
GGCAGAGACCTTGAAGTGAA
|
| Calponin 1 |
forward |
CAATGTGGGAGTCAAGTATG
|
170 bp |
| reverse |
TACCCAGTTTGGGATCATAG
|
| Caldesmon 1 |
forward |
GGTGATTTAGTTAGCCATTCG
|
276 bp |
| reverse |
TGTCAGATCTACTCACCAAG
|
| Vinculin |
forward |
ACCTGTGTCAGCCTGTTAGC
|
243 bp |
| reverse |
GGATGAAAAATGCTTGGTTG
|
