Table 3. A new line of floxed Poldip2 mice produced homozygotes as expected.

	Observed counts		Observed total	Expected total
genotype	male	female		1:2:1
WT	15	12	27	27.75
Het	32	28	60	55.50
Hom	12	12	24	27.75
Total	59	52	111	111

A new mouse colony, derived from ES cell clone B11-G2, was established as described in Fig 1. The founders did not carry the previous duplication, as shown by genotyping using the method described in Fig 5C. Furthermore, in contrast to Table 2, genotype counts of pups from Het x Het crosses were not different from a Mendelian 1:2:1 distribution, with the expected number of homozygotes, P = 0.64.