KEY RESOURCES TABLE
| REAGENT or RESOURCE Antibodies | SOURCE | IDENTIFIER |
|---|---|---|
| APC/Cy7 anti-mouse CD3 (17A2) | BioLegend | Cat# 100221; RRID:AB_2057374 |
| BV-421 CD45 (30-F11) | BioLegend | Cat# 103133; RRID:AB_10899570 |
| PE anti-mouse F4/80 (BM8) | BioLegend | Cat# 123109; RRID:AB_893498 |
| PE anti-mouse Foxp3 (FJK-16 s) | Invitrogen | Cat# 12-5773-80; RRID:AB_465935 |
| APC/Cy7 anti-mouse CD11b (M1/70) | BioLegend | Cat# 101225; RRID:AB_830641 |
| PE anti-mouse CD80 (16-10A1) | BioLegend | Cat# 104733; RRID:AB_2563112 |
| PerCP/Cy5.5 anti-mouse CTLA4 (UC10-4B9) | BioLegend | Cat# 106315; RRID:AB_2564473 |
| APC/Cy7 anti-mouse MHCII I-A/I-E (M5/114.15.2) | BioLegend | Cat# 107627; RRID:AB_1659252 |
| PE anti-mouse Gr1 (RB6-8C5) | BioLegend | Cat# 108407; RRID:AB_313372 |
| APC anti-mouse Ly6G (1A8) | BioLegend | Cat# 127613; RRID:AB_1877163 |
| APC anti-mouse CD206 (C068C2) | BioLegend | Cat# 141707; RRID:AB_10896057 |
| Mouse BD Fc Block (2.4G2) | BD pharmingen | Cat# 553142; RRID:AB_394657 |
| PE mouse anti-mouse T-Bet (4B10) | BD pharmingen | Cat# 561265; RRID:AB_10565980 |
| APC anti-mouse CD4 (GK1.5) | BioLegend | Cat# 100411; RRID:AB_312696 |
| PE/Cy7 CD8 (53-6.7) | BioLegend | Cat# 100721; RRID:AB_312760 |
| PerCP/Cy5.5 anti-mouse CD8 (53-6.7) | BioLegend | Cat# 100733; RRID:AB_2075239 |
| PerCP/Cy5.5 anti-mouse CD86 (GL-1) | BioLegend | Cat# 105027; RRID:AB_893420 |
| PerCP/Cy5.5 Ep-CAM (G8.8) | BioLegend | Cat# 107627; RRID:AB_1659252 |
| PerCP/Cy5.5 anti-mouse PD1 (29F.1A12) | BioLegend | Cat# 135207; RRID:AB_10550092 |
| Pe/Cy7 anti-mouse IFN-g (XMG1.2) | BioLegend | Cat# 505825; RRID:AB_1595591 |
| PE anti-mouse CD31 (390) | BioLegend | Cat# 102407; RRID:AB_312902 |
| Pe/Cy7 anti-mouse PDGFRa (APA5) | BioLegend | Cat# 135911; RRID:AB_2715973 |
| PE anti-mouse CD11c (N418) | BioLegend | Cat# 117307; RRID:AB_313776 |
| PE IgG1k isotype control | Invitrogen | Cat# 12-4301-81; RRID:AB_470046 |
| Pe/Cy7 Rat IgG1 isotype control | BioLegend | Cat# 400415; RRID:AB_326521 |
| InVivo rat IgG2a mAb (2A3) isotype control | BioXCell | Cat# BE0089; RRID:AB_1107769 |
| InVivoMAb monoclonal anti-mouse Ly6G (1A8) | BioXCell | Cat# BE0075-1; RRID:AB_1107721 |
| InVivoMAb CD40 rat anti-mouse IgG2a mAb (FGK45) | BioXCell | Cat# BE0016-2; RRID:AB_1107647 |
| InVivoMAb anti-rat Kappa Immunoglobulin Light Chain | BioXCell | Cat# BE0122; RRID:AB_10951292 |
| APC anti-mouse CD34 (HM34) | BioLegend | Cat #128611; RRID:AB_10553895 |
| MPO antibody | Santa Cruz | Cat# sc-390109; RRID:AB_2892996 |
| Neutrophil Marker (NIMP-R14) antibody | Santa Cruz | Cat# sc-59338; RRID:AB_2167795 |
| P53 (1C12) Mouse antibody | Cell Signaling | Cat# 2524S; RRID:AB_331743 |
| Anti-Vinculin mouse antibody | SigmaAldrich | Cat# V9264; RRID:AB_10603627 |
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| Bacterial and virus strains | ||
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| STBL3 | ThermoFisher | Cat# C737303 |
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| Chemicals, Peptides, and Recombinant Proteins | ||
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| Nab-paclitaxel | Celgene | Cat# 68817-134-50 |
| Gemcitabine | Hospira | Cat# 0409-0181-01 |
| Albumin | Sigma Aldrich | Cat# A5843 |
| Xtremegene 9 | Roche | Cat# 06 365 779 001 |
| Polybrene | Sigma Aldrich | Cat# TR-1003-G |
| Hygromycin | Sigma Aldrich | Cat# 400053 |
| G418 | Sigma Aldrich | Cat# 345812 |
| PfIFI | New England Biolabs | Cat# R0595S |
| BsmBI | New England Biolabs | Cat#R0739S |
| Shield-1 | Cheminpharma | Cat# S1-001 |
| Lipofectamine 3000 | Life Technologies | Cat# L3000008 |
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| Critical commercial assays | ||
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| Surveyor DNA mutation Kit | IDT | Cat# 706020 |
| TOPO TA Cloning Kit | Thermo-Fisher | Cat# K4575J10 |
| SYBR Green PCR Master Mix | Thermo-Fisher | Cat# 4309155 |
| Quantitect Reverse Transcription Kit | QIAGEN | Cat# 205311 |
| Zombie Green Fixable Viability Kit | Biolegend | Cat# 423111 |
| RNeasy Mini Kit | QIAGEN | Cat# 157029548 |
| Milliplex Mouse Cytokine Magnetic Bead Panel | Millipore Sigma | Cat# MCYTOMAG-70K-03 |
| KAPA Mouse Genotyping Kit | Roche | Cat# 07961804001 |
| Foxp3/Transcription Factor Staining Buffer Set | Invitrogen | Cat# 00-5523-00 |
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| Experimental models: Cell lines | ||
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| HEK293T | ATCC | Cat# ATCC® CRL-3216 |
| KrasG12D/+;Trp53+/+PDEC | Pylayeva-Gupta et al., 2012 | N/A |
| KrasG12D/+;Trp53−/− PDEC | This manuscript | N/A |
| KrasG12D/+;Trp53R172H/+PDEC | This manuscript | N/A |
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| Experimental models: Organisms/strains | ||
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| C57Bl6/J mice females (6-10weeks) | Charles River Laboratories | CR: 632 |
|
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| Oligonucleotides | ||
| p53 sgRNA, CGG AGC AGC GCT CAT GGT GG | This manuscript | N/A |
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| p53 HDR template, CAC CTC CAG CTG GGA GCC GTG TCC GCG CCA TGG CCA TCT ACA AGA AGT CAC AGC ACA TGA CGG AAG TCG TGA GAC ACT GTC CCC ACC ATG AGC GCT GCT CCG ATG GTG ATG GTA AGC CCT CAA CAC CGC CTG TGG GGT TAG GAC TGG CAG | This manuscript | N/A |
| p53 Forward PCR primer, TGGGA CAGCCAAGTCTGTTA | This manuscript | N/A |
| p53 Reverse PCR primer CCTGCT GTCTCCAGACTCCT | This manuscript | N/A |
| shScramble CCGGTCCTAAGGTTA AGTCGCCCTCGCTCGAGCGAGGG CGACTTAACCTTAGGTTTT | Sarbassov et al., 2005 | Addgene Cat # 1864 |
| CXCL2 forward PCR primer ATGC CTGAAGACCCTGCCAAG | Roy et al., 2012 | N/A |
| CXCL2 reverse PCR primer GGTC AGTTAGCCTTGCCTTTG | Roy et al., 2012 | N/A |
| CXCL5 forward PCR primer ACAGT GCCCTACGGTGGAAGT | Roy et al., 2012 | N/A |
| CXCL5 reverse PCR primer CGAGT GCATTCCGCTTAGCTT | Roy et al., 2012 | N/A |
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| Recombinant DNA | ||
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| pLKO.1 neo | Gift from Sheila Stewart | Addgene Cat# 13425 |
| psPAX2 | Gift from Didier Trono | Addgene Cat# 12260 |
| pMD2G | Gift from Didier Trono | Addgene Cat# 12259 |
| pLKO.1 hygro | Gift from Bob Weinberg | Addgene Cat# 24150 |
| LentiCRISPRv2 hygro | Stringer et al., 2019 | Addgene Cat# 98291 |
| conditional EDCPV | Gift from R. Sordella, Cold Spring Harbor Laboratory | N/A |
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| Software and algorithms | ||
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| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/; RRID:SCR_001935 |
| FlowJo version 10.2 | FlowJo | https://www.flowjo.com/solutions/flowjo/downloads; RRID:SCR_008520 |
| R version 3.4.3 | RStudio | http://www.R-project.org; RRID:SCR_000432 |
| Prism Version 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| Vevo Lab | FUJIFILM-VisualSonics | https://www.visualsonics.com/product/software/vevo-lab |
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| Other | ||
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| Whole-Tissue Gene Expression Data of Pancreatic Ductal Adenocarcinoma | Badea et al., 2008 | GSE15471 |
| Expression data from Mayo Clinic Pancreatic Tumor and Normal samples | Pei et al., 2009 | GSE16515 |