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. 2021 Dec 6;12:754554. doi: 10.3389/fphar.2021.754554

TABLE 1.

qRT-PCR amplification primer.

Target gene Upstream primer (5′–3′) Downstream primer (5′–3′)
TNF–α TAT​GGC​TCA​GGG​TCC​AAC​TC GCT​CCA​GTG​AAT​TCG​GAA​AG
NF–kB ACG​ATC​TGT​TTC​CCC​TCA​TCT TGG​GTG​CGT​CTT​AGT​GGT​ATC
IL–1β TCC​AGG​ATG​AGG​ACA​TGA​GCA​C GAA​CGT​CAC​ACA​CCA​GCA​GGT​TA
IL–6 CAA​AGC​CAG​AGT​CCT​TCA​GAG GTC​CTT​AGC​CAC​TCC​TTC​TG
Klotho GGG​ACA​CTT​TCA​CCC​ATC​ACT TGG​GTG​CGT​CTT​AGT​GGT​ATC
GAPDH ACA​GTC​CAT​GCC​ATC​ACT​GCC GCC​TGC​TTC​ACC​ACC​TTC​TTG