FIG. 11.

hym1Δ and cbk1Δ mutants have similar phenotypes, and Hym1p is required for normal Cbk1p localization. (A) Morphology of vegetatively growing and pheromone-treated wild-type (WT) and cbk1Δ, hym1Δ, and cbk1Δ hym1Δ mutant cells. Exponentially growing diploid cells were fixed with formaldehyde and photographed. Bar, 10 μm. Exponentially growing haploid cells were treated with α-factor at 5 μg/ml for 2 h, fixed, and then photographed. Bar, 5.0 μm. (B) Cbk1p::3xHA was visualized by indirect immunofluorescence assay in wild-type and hym1Δ mutant cells. No polarized localization of Cbk1p::3xHA is observed in hym1Δ mutant cells. Bar, 5.0 μm.