| Strain, strain background |
Escherichia coli TOP10 |
Invitrogen |
|
See Supplementary file 1b
|
| Strain, strain background |
Campylobacter jejuni NCTC11168 |
Arnoud van Vliet, Institute of Food Research, Norwich, UK |
|
See Supplementary file 1b
|
| Sequence-based reagent |
(Oligonucleotides)See Supplementary file 1c
|
Sigma |
|
See Supplementary file 1c
|
| Recombinant DNA reagent |
(Plasmids)See Supplementary file 1d
|
This study |
|
See Supplementary file 1d
|
| Antibody |
Anti-FLAG M2 (mouse monoclonal) |
Sigma-Aldrich |
#F1804-1MG |
Western blot 1:10,000 |
| Antibody |
Anti-GFP (mouse monoclonal) |
Roche |
#11814460001 |
Western blot 1:1000 |
| Antibody |
Anti-GroEL (rabbit polyclonal) |
Sigma-Aldrich |
#G6532-5ML |
1:10,000 |
| Antibody |
Anti-mouse HRP conjugate IgG (sheep polyclonal) |
GE Healthcare |
#RPN4201 |
1:10,000 |
| Antibody |
Anti-rabbit HRP conjugate IgG (goat polyclonal) |
GE Healthcare |
#RPN4301 |
1:10,000 |
| Commercial assay or kit |
PURExpress |
New England Biolabs |
E6800S |
|
| Commercial assay or kit |
DNA Cycle Sequencing Kit |
Jena Bioscience |
#PCR-401S |
|
| Commercial assay or kit |
MEGAscript T7 kit (Ambion) |
Thermo Fisher Scientific |
AMB13345 |
|
| Software, algorithm |
RNAfold |
Lorenz et al., 2011 (PMID:22115189) |
Vienna RNA package 2.4.14 |
http://www.tbi.univie.ac.at/RNA
|
| Software, algorithm |
IntaRNA |
Mann et al., 2017 (PMID:28472523) |
Version 3.2.0 linking Vienna RNA package 2.4.14 |
http://rna.informatik.uni-freiburg.de/IntaRNA/Input.jsp
|
| Software, algorithm |
AIDA |
Raytest, Germany |
Version 5.0 SP1 Build 1,182 |
|
| Software, algorithm |
Integrated Genome Browser |
UNC Charlotte |
Version 9.1.8 |
bioviz.org
|