| Antibodies |
|
| anti-GAPDH |
Life Technologies |
Cat# AM4300, RRID:AB_437392
|
| anti-p24 antibody (AG3.0) |
AIDS respository |
Cat# ARP4121 |
| Anti-spike antibody |
GeneTex |
Cat# GTX632604, RRID:AB_2864418
|
| goat anti-mouse HRP-conjugated second antibody |
Sigma |
Cat# A4416, RRID:AB_258167
|
| Alexa-fluor 594-conjugated goat anti-mouse IgG |
Biolegend |
Cat# 405326, RRID:AB_2563308
|
|
| Bacterial and virus strains |
|
| SARS-CoV-2 Δ19 S (D614G, Beta, Delta, Delta+N501S, Mu, C.1.2) pseudotyped reporter virus |
This paper |
N/A |
|
| Biological samples |
|
| Convalescent sera |
NYU Vaccine Center with written consent under I.R.B. approval |
IRB 20-00595 and IRB 18-02037 |
| Sera from BNT162b2, mRNA-1273, AD26.COV2.S vaccinated donors |
NYU Vaccine Center with written consent under I.R.B. approval |
IRB 20-00595 and IRB 18-02037 |
| Sera from BNT162b2 vaccinated donors (Previously infected) |
NYU Vaccine Center with written consent under I.R.B. approval |
IRB 20-00595 and IRB 18-02037 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Soluble ACE2-NLuc |
This paper |
N/A |
| Soluble ACE2 |
Tada et al., 2020 |
N/A |
| REGN10933 |
Tada et al., 2021 |
N/A |
| REGN10987 |
Tada et al., 2021 |
N/A |
|
| Critical commercial assays |
|
| Nano-Glo® Luciferase Assay System |
Promega |
Cat# N1120 |
| Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate |
Applied Biosystems |
Cat# T1056 |
|
| Experimental models: Cell lines |
|
| 293T |
ATCC |
N/A |
| ACE2.293T |
Tada et al., 2020 |
N/A |
| ACE2.A549 |
Tada et al., 2021 |
N/A |
|
| Software and algorithms |
|
| MacVector ver 17.0.0 (27) |
MacVector Inc |
N/A |
| GraphPad Prism 8 Software |
GraphPad Prism Software, Inc. |
N/A |