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. 2021 Dec 21;38(2):110237. doi: 10.1016/j.celrep.2021.110237
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

anti-GAPDH Life Technologies Cat# AM4300, RRID:AB_437392
anti-p24 antibody (AG3.0) AIDS respository Cat# ARP4121
Anti-spike antibody GeneTex Cat# GTX632604, RRID:AB_2864418
goat anti-mouse HRP-conjugated second antibody Sigma Cat# A4416, RRID:AB_258167
Alexa-fluor 594-conjugated goat anti-mouse IgG Biolegend Cat# 405326, RRID:AB_2563308

Bacterial and virus strains

SARS-CoV-2 Δ19 S (D614G, Beta, Delta, Delta+N501S, Mu, C.1.2) pseudotyped reporter virus This paper N/A

Biological samples

Convalescent sera NYU Vaccine Center with written consent under I.R.B. approval IRB 20-00595 and IRB 18-02037
Sera from BNT162b2, mRNA-1273, AD26.COV2.S vaccinated donors NYU Vaccine Center with written consent under I.R.B. approval IRB 20-00595 and IRB 18-02037
Sera from BNT162b2 vaccinated donors (Previously infected) NYU Vaccine Center with written consent under I.R.B. approval IRB 20-00595 and IRB 18-02037

Chemicals, peptides, and recombinant proteins

Soluble ACE2-NLuc This paper N/A
Soluble ACE2 Tada et al., 2020 N/A
REGN10933 Tada et al., 2021 N/A
REGN10987 Tada et al., 2021 N/A

Critical commercial assays

Nano-Glo® Luciferase Assay System Promega Cat# N1120
Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate Applied Biosystems Cat# T1056

Experimental models: Cell lines

293T ATCC N/A
ACE2.293T Tada et al., 2020 N/A
ACE2.A549 Tada et al., 2021 N/A

Software and algorithms

MacVector ver 17.0.0 (27) MacVector Inc N/A
GraphPad Prism 8 Software GraphPad Prism Software, Inc. N/A