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. 2021 Dec 13;2021:5048375. doi: 10.1155/2021/5048375

Figure 7.

Figure 7

Effects of LY294002 and Rapa on the activation of p-Akt/p-mTOR in LPS-stimulated BMECs. (a) The expression of PI3K in the presence of LY294002 was examined by immunofluorescence. (b) The protein expressions of PI3K, p-Akt/Akt, and p-mTOR/mTOR in the presence of LY294002 were examined by western blotting. (c) The protein levels of p-mTOR/mTOR in the presence of Rapa were examined by western blotting. (d) The mRNA levels of IL-6, IL-8, iNOS, and COX-2. (e) The mRNA levels of ZO-1 and Occludin. (f) The apoptosis rate. (g) The formation of apoptotic bodies. (h) The ratio of Bcl-2/Bax. All results are presented as the means ± SEM (n = 3). Significance compared with control, ∗P < 0.05 and∗∗P < 0.01. Within the LPS-treated groups, significance compared with LPS group, #P < 0.05, ##P < 0.01, &P < 0.05, and &&P <0.01.