Table 1.
Primer sequences.
| Genes | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|
| In vitro | ||
| IL-1β | GGCAACCGTACCTGAACCCA | CCACGATGACCGACACCACC |
| IL-6 | CCTTCACTCCATTCGCTGTCT | TCCTGATTTCCCTCATACTCG |
| IL-8 | ATGACTTCCAAGCTGGCTGTT | GGTTTAGGCAGACCTCGTTTC |
| iNOS | GGACTTGGCTACGGAACTGG | GGTGAAGCGTGTCTTGGAAA |
| COX-2 | GGCGATGAGCAGTTGTTCCA | TGCTGTACGTAGTCTTCAATCACAAT |
| Bax | GTGCCCGAGTTGATCAGGAC | CCATGTGGGTGTCCCAAAGT |
| Bcl-2 | GAGTTCGGAGGGGTCATGTG | GCCTTCAGAGACAGCCAGGA |
| ZO-1 | GACTTGTCAGCTCAGCCAGT | GGCTCCTCTCTTGCCAACTT |
| Occludin | TTCTTCAGGCGGAGACGGC | GATGACATGGCTGGTGTCAGTG |
| Claudin1 | CCCGTGCCTTGATGGTGATT | AAGACAGCCATCCGCATCTT |
| In vivo | ||
| TNF-α | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
| IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| IL-6 | TTCTTGGGACTGATGCTGGT | AGACAGGTCTGTTGGGAGTG |
| iNOS | TGCCACGGACGAGACGGATAG | CTCTTCAAGCACCTCCAGGAACG |
| COX-2 | GGTGCCTGGTCTGATGATGTATGC | GGATGCTCCTGCTTGAGTATGTCG |
| ZO-1 | ACTCCCACTTCCCCAAAAAC | CCACAGCTGAAGGACTCACA |
| Occludin | ATTCCATCAGTTTCCTATCT | ACCAGGACCTTTCTTGAC |
| Claudin1 | AGACCTGGATTTGCATCTTGGTG | TGCAACATAGGCAGGACAAGAGTTA |