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The Gal4 binding sites in the ORF can function as UASGAL. Sites 2 and 3 were cloned into a reporter construct, pMEL-β-gal, in which the UASGAL of MEL1 gene was removed and replaced with a cloning site. W303a cells transformed with the pMEL-β-gal and pMel-ACC1(S2&S3)-β-gal were grown in glycerol-lactic acid and galactose. β-Gal activity was measured as described in the text.
