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Gal4 represses the transcription of ACC1. (A) Wild-type (WT) ACC1 mRNA levels in raffinose (lane 1) and galactose (lane 2) and mutant ACC1 mRNA levels in raffinose (lane 3) and galactose (lane 4). In the mutant ACC1, two Gal4 binding sites, sites 2 and 3, were eliminated without changing the codons.
