FIG. 7.

The Gal4 regulation of ACC1 has no effect on growth. The wild-type (WT) and mutant (S2,3−) yeast cells were mixed in a 1:1 ratio and grown in raffinose (Raf) or galactose (Gal) for 10 (lanes 2 and 6), 20 (lanes 3 and 7), or 30 (lanes 5 and 8) generations. Southern blots were performed using a ACC1 probe, and the genomic DNA was digested with HpaII. In the site 2 and 3 mutant, one HpaII site was destroyed.