Extended Data Fig. 1. In vitro screening of a natural product library with human stromal cells.
(a) Schematic workflow of cell-based screening strategy for a natural library of medicinal agents. (b) Quantification of cell survival of control (CTRL) and senescent (SEN) PSC27 after treatment with ABT-263 (1.25 μM), ABT-737 (10 μM) or Dasatinib (250 nM) combined with Quercetin (50 μM) for 3 d. (c) Screening results after pharmacological assays. Library agents were assessed at a concentration of 3 μg/ml with 5.0 × 103 cells for 3 d. Hits were selected based on their ability to specifically kill senescent cells. Each blue dot represents a single agent as the mean of three replicates. Red rectangular region denotes potential candidates of senolytics in the case of TIS. (d) Appraisal of the effects of randomly selected agents (23 representative candidates out of 46 in the library, each applied at 1 μg/ml) on the survival of CTRL and SEN cells. (e) Assessment of the effects of remaining agents (23 candidates out of 46 in the library, each applied at 1 μg/ml) on the survival of CTRL and SEN cells. For all datasets, samples were collected for analyses 3 days after treatment with individual agents in culture condition. TIS, therapy-induced senescence. Data in b, d and e are shown as mean ± SD and representative of 3 independent biological replicates, with P values calculated by a two-sided t-test. *, P < 0.05; **, P < 0.01.