Extended Data Fig. 10. Functions of peroxisomal β-oxidation and ROS in regulating TAG mobilization in liver.
(a) ROSA26-LSL-spCas9 mice were injected with a pool of AAV to express Cat gRNA in the liver. After 2 weeks on NCD or 8 weeks on HFD, hepatic lipids were extracted for TAG content determination (NCD N = 5 in both genotypes; HFD N = 7 in both genotypes). (b) Representative H&E staining images of livers from liver specific Cat knockout mice fed on NCD or HFD for 8 weeks. Scale bar, 100μm. Repeated 3 times. (c) Acox1fl/fl mice were injected with AAV-TBG-Cre virus to knock out Acox1 in the liver. After 2 weeks on NCD or 8 weeks on HFD, hepatic lipids were extracted for TAG content determination (NCD N = 6 in both genotypes; HFD WT N = 8 and Acox1KO N = 9). (d) Representative H&E staining images of livers from liver specific Acox1 knockout mice fed on NCD or HFD for 8 weeks. Scale bar, 100μm. Repeated 3 times. (e-h) Lipophagy/autophagy in the livers was analyzed via IB using surrogate autophagy markers, as indicated. Repeated 3 times. Results are shown as mean ± SEM and analyzed using a Student’s two-sided t test.