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. 2021 Dec 13;3(12):1648–1661. doi: 10.1038/s42255-021-00489-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–c, iBAs were transfected with Cat siRNA. After 72 h, IB, glycerol and NEFA measurement were conducted to check lipolytic proteins or lipolysis levels (n = 6 in Cat and 10 in Nc siRNA, F = 22 (a); n = 5, F = 13.74 (b) and 16.12 (c)). d–f, iBAs were transfected with Acox1 siRNA. After 72 h, IB, glycerol and NEFA measurements were conducted to check lipolytic proteins or lipolysis levels (n = 6 in Acox1 and 8 in Nc siRNA, F = 16.74 (d); n = 5, F = 16.6 (e) and 35.79 (f)). g–i, iBAs were treated with 100 μM C26:0 and C24:0 for 48 h. IB, glycerol and NEFA measurements were conducted to check lipolytic proteins or lipolysis levels (n = 8 in VLCFAs treatment and 13 in control, F = 60.22 (g); n = 5, F = 34.4 (h) and 29.5 (i)). j–l, iBAs were treated with 500 μM H₂O₂ at the indicated time points. IB, glycerol and NEFA measurements were conducted to check lipolytic proteins or lipolysis levels (n = 4 in H₂O₂ treatment and 12 in control, F = 7.451 (j); n = 5, F = 9.331 (k) and 15.92 (l)). m–o, iBAs were treated with 1 mM, 2 mM and 3 mM NAC for 24 h. IB, glycerol and NEFA measurements were conducted to check lipolytic proteins or lipolysis levels (n = 4 in 2 mM and 3 mM NAC treatment and 5 in 0 mM and 1 mM NAC treatment, F = 17.13 (m); n = 5, F = 48.29 (n) and 16.28 (o)). Results are shown as the mean ± s.e.m. and analysed using ANOVA with Dunnett correction for multiple comparisons between control and other groups.

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