Fig. 2. Insulin uptake in tanycytes and MBH is distorted in IR^∆Tan mice.

a, Representative images of immunostaining for fluorescently labelled insulin (anti-Alexa-Fluor-488) 15 min post i.v. injection of 488-insulin (250 nmol kg⁻¹) in IR^fl/fl mice, which received either AAV-Dio2-mKate2 (IR^mKate2) control or Cre-recombinase-expressing AAV-Dio2-Cre virus (IR^ΔTan). 488-insulin was quantified in DAPI-positive tanycyte layer. White and yellow dashed lines indicate quantified ROI in basal and lateral tanycytes, respectively. b–d, Mean intensity signal of Alexa-Fluor-488 fluorescent insulin in basal, P = 0.013, (b) and lateral (c) tanycytes and ARC, P = 0.0251, (d) of IR^mKate2 and IR^ΔTan mice. e, Quantification of Alexa-Fluor-488-positive cells per hemisphere of ARC of IR^mKate2 and IR^ΔTan mice, P = 0.0502. b,d,e, Unpaired, two-sided Student’s t-test. a–e, n = 7/IR^mKate2, 5/IR^ΔTan. Data are represented as the mean ± s.e.m. Scale bar, 100 µm.

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