Fig. 3. Tanycyte but not endothelial IR is necessary to maintain insulin sensitivity and rebound food intake.

a,e, Body weight change of IR^GFP-Tan and IR^ΔTan mice normalized to 1 week post i.c.v. injection (13 weeks) (a) and IR^wt/wt and IR^ΔBVEC mice normalized to 1 week post tamoxifen treatment (11 weeks) (e), n = 13/IR^GFP-Tan, IR^wt/wt; n = 14/IR^ΔTan; n = 15/IR^ΔBVEC. a, Two-way ANOVA P = 0.0064, P(17 w) = 0.0055, P(18 w) = 0.0046, P(19 w) = 0.0008 (two-way ANOVA, Šídák post hoc test). b,f, Rebound food intake after 16-h fasting of IR^GFP-Tan and IR^ΔTan mice, P(0–1 h) = 0.028, (b) and IR^wt/wt and IR^ΔBVEC mice (f); n = 16/IR^GFP-Tan, IR^ΔTan; n = 17/IR^wt/wt, IR^ΔBVEC (unpaired, two-sided Student’s t-test). c,g, Insulin tolerance test and area under the curve (AUC) of IR^GFP-Tan and IR^ΔTan mice (c) and IR^wt/wt and IR^ΔBVEC mice (g), n = 13/IR^GFP-Tan, IR^wt/wt; n = 14/IR^ΔTan; n = 15/IR^ΔBVEC, c, Two-way ANOVA P = 0.0334, P(30 min) = 0.0327, P(60 min) = 0.0318 (two-way ANOVA, Šídák post hoc test), P(AUC) = 0.031. d,h, Glucose tolerance test and AUC of IR^GFP-Tan and IR^ΔTan mice (d) and IR^wt/wt and IR^ΔBVEC mice (h), n = 13/IR^GFP-Tan, IR^wt/wt; n = 14/IR^ΔTan; n = 15/IR^ΔBVEC. a–h, Data are represented as the mean ± s.e.m. w, weeks.

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