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. 2021 Sep 23;13(2):425–440. doi: 10.1016/j.jcmgh.2021.09.009

Figure 1.

Figure 1

SIGIRR is required for anti-inflammatory microRNA expression in HIECs. (A) HIEC lysates of nontransfected(C, control), transfected with Scramble siRNA and SIGIRR siRNA were analyzed by Western blot using the indicated antibodies, with densitometry quantification shown graphically. (B) microRNA expression was quantified by real-time PCR in HIECs transfected with scramble or SIGIRR siRNA. (C) HIECs were transfected with pcDNA3–empty vector (EV), pcDNA3–SIGIRR, and pcDNA3–SIGIRR p168X mutant, and the cell lysates were analyzed by Western blot using the indicated antibodies. The densitometry quantification is shown graphically. (D) microRNA expression was quantified by real-time PCR in HIECs with indicated transfection. ∗P < .05, #P < .05, ∗∗P < .01, ∗∗∗P < .005. ACTB, actin beta.