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. 2021 Sep 23;13(2):425–440. doi: 10.1016/j.jcmgh.2021.09.009

Figure 6.

Figure 6

Decreased intestinal expression of microRNAs and increased TLR signaling molecules in SigirrTg mice. (A) Immunofluorescent confocal images of DOL-11 mouse terminal ileum stained with phospho-STAT3 (Ser727) antibody. (B) Immunofluorescent confocal images of isolated IECs from DOL-11 mouse terminal ileum stained with epithelial cell markers. (C) Sigirr mRNA expression in isolated IECs. (D) microRNA expression in IECs isolated from terminal ileum of DOL-12 SigirrTg mice was quantified by real-time PCR. (E) Mouse IEC lysates from littermate SigirrTg mice were analyzed by immunoblotting with indicated antibodies, with densitometry quantification shown graphically. (F) Immunofluorescent confocal images of DOL-11 mouse terminal ileum stained with IRAK1 antibody. ∗P < .05, ∗∗P < .01, ∗∗∗P < .005, ∗∗∗∗P< .001. ACTB, actin beta; DAPI, 4′,6-diamidino-2-phenylindole;