Fig. 3. CMA-defective mice display disturbances in circadian patterns.

a, Immunostaining for BMAL1, PER1, CRY1 and REVERBα in the suprachiasmatic nucleus region (SCN) of brains of wild-type (WT) or LAMP2A knockout mice (L2A^KO) at the indicated Zeitgeber times (ZT). b, Quantification of the average fluorescent intensity per cell in the SCN area in images as the ones shown in a. n=3 mice per ZT and genotype. c, Double-plotted actograms of WT and L2A^KO mice subjected to the light manipulations indicated in the middle for the number of days indicated on the left. LD, 12:12 light:dark; DD, continuous dark; LL, continuous light. d, Representative Morlet Wavelet of WT and L2A^KO mice under the same conditions as in c. e, Average free running circadian period in the DD (left) and LL (right) cycles. n=9 WT mice per condition and 9 (for DD) and 8 (for LL) L2A^KO mice. f, Representative activity profile of WT and L2A^KO mice for an average 10 cycle in DD (left) or LL (right). The red line is a sine-fit to the waveform indicating the rhythm of these mice. Individual values (e) and mean±s.e.m are shown. Unpaired two-tailed t test was used in e and two-way ANOVA test followed by Bonferroni’s multiple comparisons test post-hoc test was used for b. Significant differences by genotype across the ZT are shown in the legends. Differences were significant for *p<0.05, **p<0.01 and ****p<0.0001. ns = not significant. Numerical source data, statistics and exact p values are available as source data.