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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Head Neck. 2021 Oct 25;44(1):177–188. doi: 10.1002/hed.26906

Figure 3. High expression of NFX1-123 in HPV positive OPC HNSCC cell lines.

Figure 3.

A. Quantitative PCR analysis of NFX1-123 was performed to determine the mRNA expression and was normalized to 36B4 as an internal control. Differential NFX1-123 expression between HPV negative and positive HNSCC cell lines was determined. B. Western blot analysis of NFX1-123 in HPV negative and positive HNSCC cell lines. HPV positivity was confirmed with p16 expression. p53, a protein degraded by HPV16 E6, was not detected in HPV positive cells. HN4 HPV negative cells do not express p53 due to a splice site mutation in these cells. GAPDH was used as a loading control. ImageJ analysis of NFX1-123 expression, normalized to GAPDH, is shown.