KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-γH2AX | GeneTex | GTX127342; RRID:AB_2833105 |
| Rabbit anti Phospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) | Cell Signaling Technology | CST-4370S; RRID:AB_2895021 |
| Goat anti-rabbit IgG H&L (Alexa FluorTM 594) | Invitrogen | A11012; RRID:AB_141359 |
| Goat anti-rabbit IgG H&L (Alexa FluorTM 488) | Invitrogen | A11008; RRID:AB_143165 |
| DAPI/Antifade (250 ng/ml) counterstain | Santa Cruz Biotechnology | sc-3598 |
| Parp1 Antibody | Abcam | ab194586; RRID:AB_2895023 |
| Anti-GAPDH Antibody (0411) | Santa Cruz Biotechnology | sc-47724; RRID:AB_627678 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Etoposide (ETO) | SIGMA-ALDRICH | E1383 |
| NU1025 | SIGMA-ALDRICH | N7287 |
| Pentylenetetrazole (PTZ) | SIGMA-ALDRICH | P6500 |
| Dimethyl Sulfoxide (DMSO) | SIGMA-ALDRICH | D8418 |
| Paraformaldehyde (PFA), prills, 95% | SIGMA-ALDRICH | 441244 |
| Polyethylene glycol 200 (PEG-200) | SIGMA-ALDRICH | 8.07483 |
| Dulbecco’s Phosphate Buffered Saline (PBS), 10X Conc., without Calcium and Magnesium | Biological Industries | 02-023-5A |
| Fetal Bovine Serum (FBS) | Biological Industries | 041271A |
| Alt-R® S.p. Cas9 Nuclease V3, 100 μg | IDT | 1081058 |
| Experimental Models: Organisms/Strains | ||
| Zebrafish: tg(HuC:Gal4) | (Plucińska et al., 2012) | N/A |
| Zebrafish: tg(dbh:Gal4) | Current work | N/A |
| Zebrafish: tg(fli1:EGFP) | (Lawson and Weinstein, 2002) | N/A |
| Zebrafish: tg(sox10:EGFP) | ZFIN | ZDB-TGCONSTRCT-150414-3 |
| Zebrafish: tg(uas:DsRed-Rad52) | Current work | N/A |
| Zebrafish: tg(uas:DsRed-TRF1) | Current work | N/A |
| Zebrafish: tg(HuC:GCaMP5) | (Romano et al., 2017) | N/A |
| Zebrafish: tg(ChR2-mCherry) | (Helmbrecht et al., 2018) | N/A |
| Zebrafish: tg(uas:Lap2β-EGFP) | Current work | N/A |
| Mice: C57BL/6j | Envigo/ in house breeding | N/A |
| Oligonucleotides | ||
| Primers for cloning and RT-PCR (Methods details) | Current work | N/A |
| sgRNA list (Method details) | Current work | N/A |
| Recombinant DNA | ||
| Plasmid: pT2-uas:DsRed-Rad52 | Current work | N/A |
| Plasmid: pT2-uas:dsRed-Rad52R49A | Current work | N/A |
| Plasmid: pT2-uas:Ku80-EGFP | Current work | N/A |
| Plasmid: pT2-uas:ku80D721A/D722A-EGFP | Current work | N/A |
| Plasmid: pT2-uas:Lap2β-EGFP | (Zada et al., 2019) | N/A |
| Plasmid: pT2-uas:Lap2βLEMdel-EGFP | Current work | N/A |
| Plasmid: pT2-hsp70:Parp1 | Current work | N/A |
| Software and Algorithms | ||
| EthoVision XT 12 software | Noldus Information Technology | N/A |
| MATLAB | Mathworks | https://www.mathworks.com/products/matlab.html |
| Imaris | Oxford Instruments | N/A |
| ImageJ | NIH imageJ | https://imagej.nih.gov/ij/ |
| Excel | Microsoft | https://office.microsoft.com/excel/ |
| Other | ||
| Noldus DanioVision tracking system | Noldus Information Technology | N/A |
| CFX96™ Real-Time PCR | BIO RAD | N/A |
| Direct-zol RNA MiniPrep kit | Zymo Research Corporation | ZR-R2051 |
| qScript cDNA SuperMix | Quanta BioSciences | 95047 |
| PerfeCTa SYBR Green FastMix | Quanta BioSciences | 95073 |
| Comet assay kit | Abcam | ab238544 |