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. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Oncogene. 2021 Nov 19;40(50):6772–6785. doi: 10.1038/s41388-021-02117-5

Figure 5. Mitotic phosphorylation of PKR is required for cancer cell growth and paclitaxel chemosensitivity.

Figure 5

(A) Establishment of cell lines with exogenous PKR-WT or PKR-3A (S83A/S456A/S542A) in SKOV3-PKR-KO cells.

(B, C) Cell proliferation curves and cell migration assays (Transwell assay) with cell lines established in A.

(D) Establishment of cell lines with exogenous PKR-WT or PKR-3A (S83A/S456A/S542A) in MCF-7-PKR-KO cells.

(E-G) Cell proliferation curves and cell migration assays (Transwell assay) with cell lines established in D. Representative images of cell migration were shown in G.

(H) Cell lines from A and D were treated with DMSO or Taxol (100 nM) for 24 h. Total cell lysates were probed with cleaved-PARP to determine apoptosis.

(I, J) Cell lines from A and D were treated with Taxol (100 nM) for 24 h and the caspase 3/7 activities in these cells were analyzed by Caspase-Glo 3/7 Assay. Data in panels B, C, E, F, I, and J are expressed as mean ± SEM from three biological replicates. *: p<0.05, **: p<0.01, ***: p<0.001 (unpaired Student’s t-test).