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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Mol Microbiol. 2021 Nov 20;116(6):1489–1511. doi: 10.1111/mmi.14840

Figure 4.

Figure 4.

Low affinity adenosine transport. A. Inhibition of transport of 20 µM [3H]-adenosine by unlabelled adenosine and cytidine; incubation time was 30 s. Cytidine: EC50 = 290 µM, Hill slope −1.06, r2 = 0.96; Adenosine: EC50 = 60.5 µM, Hill slope −0.83, r2 = 0.98 for this experiment. Inset: Conversion of adenosine inhibition data to Michaelis-Menten plot, Km,app = 63.5 µM, r2 = 0.99. B. Inhibition of 20 µM [3H]-adenosine by unlabelled thymidine and uridine. Thymidine: EC50 = 817 µM, Hill slope −1.39, r2 = 0.99; Uridine: EC50 = 519 µM, Hill slope −1.21, r2 = 0.97.