Fig. 1. The comparison of bEnd5 after 24 h of either normoxia or OGD shows a significantly higher rate of cell death under OGD conditions. The MCC950 dilution series within the bEnd5 cell culture favors the 100 µmol/l MCC950 concentration with a significant reduction of cell death under OGD.

a Number of apoptotic bEnd5 cells per 0.1 cm² after 24 h of either normoxia (blue) or OGD (3.0% O₂, 5.0% CO₂, 95% humidity, 37.0 °C, 1 g/l glucose, red) depending on the concentration of the initial MCC950 treatment in comparison to untreated control cells (n = 15 out of three independent experiments). #: counting stopped at 1000 dead cells/0.1 cm². b Representative microscopic brightfield images of bEnd5 after 24 h OGD and immunofluorescent staining of the propidium iodide (red) uptake of these bEnd5 cells as cell death marker in selected MCC950 concentrations. In all, ×20 objective; scale bar = 25 µm. Data were analyzed by one-way ANOVA with Tukey post hoc test. **p < 0.01; ***p < 0.001.