Fig. 4. bEnd5 express less pro-inflammatory chemokines and cytokines after MCC950 (100 µmol/l) treatment.

a–d Cytometric bead array to detect endothelial chemokine release depending on OGD (3.0% O₂, 5.0% CO₂, 95% humidity, 37.0 °C, 1 g/l glucose, red) duration and treatment regime. a CXCL1 protein concentration (n = 6 out of three independent experiments), b CCL2 (MCP-1) protein concentration (n = 6 out of three independent experiments), c CCL5 (RANTES) protein concentration (n = 6 out of three independent experiments) and d CXCL10 (IP10) protein concentration (n = 6 out of three independent experiments) at baseline under normoxic conditions as well as after 5 h, 10 h, and 24 h of OGD with vehicle or MCC950 treatment. e–f ELISA to measure endothelial cytokine release depending on OGD duration and treatment regime. e IL1b protein concentration (n = 8 out of four independent experiments), f IL18 protein concentration (n = 8 out of four independent experiments). Data were analyzed by unpaired t test. *p < 0.05; **p < 0.01; ***p < 0.001.