Fig. 5. MMP9 reduction and ZO-1 enhancement after MCC950 (100 µmol/l) treatment.

a Top: representative zymography with Coomassie staining depicting the amounts of secreted MMP9 (92 kDa) in bEnd5 supernatants after 5 h, 10 h, or 24 h of normoxia (blue) or OGD (3.0% O₂, 5.0% CO₂, 95% humidity, 37.0 °C, 1 g/l glucose, red) either with vehicle or MCC950 (100 µmol/l) treatment. Bottom: the intensity of MMP9 within the bEnd5 supernatants were normalized to the intensity of the probes at 0 h under normoxic conditions (dashed line, n = 7 out of three independent experiments). Data were analyzed by unpaired t test. *p < 0.05. ***p < 0.001. b Intensities of ZO-1 immunohistochemical stainings of bEnd5 under normoxia or OGD with either vehicle or MCC950 (100 µmol/l) treatment. Data were analyzed by one-way ANOVA with Tukey post hoc test. **p < 0.01; ***p < 0.001. c Representative ZO-1 (green) and DAPI (blue) immunofluorescence stainings of bEnd5 after 24 h of OGD either with vehicle or with MCC950 (100 µmol/l) treatment. ×20 objective; scale bar = 50 µm.