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. 2021 Dec 20;13(1):15. doi: 10.1038/s41419-021-04387-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a The qPCR-based expression levels of miR-17-5p and miR-93-5p upon MYC knockdown. b ChIP analysis of MYC enrichment at the promoters of miR-17 and miR-93 genes in PANC-1 cells. Western blotting was performed to detect MYC protein after immunoprecipitation, where IgG was used as a negative control. An isotype-matched IgG antibody was used as a control in ChIP analysis. c ChIP analysis of MYC enrichment at the promoters of the miR-17 and miR-93 genes in control and MYEOV-silenced PANC-1 cells. ChIP values are expressed as the means ± SEM of relative input chromatin (%). An isotype-matched IgG antibody was used as a control. d Association of FLAG-tagged MYEOV with HA-tagged MYC as detected by immunoprecipitation. e Endogenous interaction between MYEOV and MYC as revealed by co-immunoprecipitation. f Endogenous interaction between MYEOV and MYC as detected by immunofluorescence. MYEOV (Red); MYC (Green); Nucleus (Blue). All n = 3; bar, SEM; *P < 0.05, **P < 0.01 and ***P < 0.001; Student’s t-test.