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. 2021 Dec 20;13(1):12. doi: 10.1038/s41419-021-04464-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HepG2, SK-Hep1, and SMMC-7721 cells were transfected with NC, miR-541-5p mimics, and miR-541-5p inhibitor. A–C Expression of miR-541-5p in HepG2, SK-Hep1, and SMMC-7721 cells. D–F Cell proliferation was detected in HepG2, SK-Hep1, and SMMC-7721 cells using CCK-8 assay. G–I Effect of miR-541-5p on migration and invasion were assessed using transwell migration and invasion assays; Scale bar, 200 μm. J–L Cells were stained with Annexin V-APC and PI, and the percentage of apoptotic cells was determined through flow cytometry. Data were presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01.