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. 2021 Dec 20;13(1):12. doi: 10.1038/s41419-021-04464-3

Fig. 4. CircMTO1 regulated miR-541-5p and affected the proliferation, migration, invasion, and apoptosis of HCC cells.

Fig. 4

HepG2, SK-Hep1, and SMMC-7721 cells were transfected with NC, circMTO1 siRNAs, and/or miR-541-5p inhibitor. A–C Analysis of circMTO1 expression using RT-qPCR after transfection with circMTO1 siRNAs. D–F Cell proliferation was determined using CCK-8 assay. G–I The migration and invasion of HepG2, SK-Hep1, and SMMC-7721 cells were evaluated using transwell assays. Scale bar; 200 μm. J–L Cells were stained with Annexin V-APC and PI, and the percentage of apoptotic cells was measured using flow cytometry. Data were presented as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01.