Skip to main content
. 2021 Dec 20;13(1):12. doi: 10.1038/s41419-021-04464-3

Fig. 5. ZIC1 was directly targeted by miR-541-5p.

Fig. 5

A, miRDB, TargetScan, and ENCORI databases were used to predict potential mRNA targets of miR-541-5p. B The screening process of miR-541-5p target candidate. C The co-expression of miR-541-5p and ZIC1 as predicted by ENCORI in HCC. D Schematic illustration of ZIC1 3′UTR-Wt and 3′UTR-Mut luciferase reporter vectors. E, F Relative ZIC1 mRNA and protein levels were detected in cells using RT-qPCR and western blot, respectively, after transfection with miR-NC, miR-541-5p mimics, and miR-541-5p inhibitor. G, H Relative ZIC1 mRNA and protein levels were detected in cells using RT-qPCR and western blot, respectively, after transfection with NC, si-circMTO1, miR-541-5p inhibitor, and si-circMTO1 + miR-541-5p inhibitor. Data were presented as mean ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.