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. 2021 Dec 20;13(1):12. doi: 10.1038/s41419-021-04464-3

Fig. 6. Modulation of ZIC1 and the effect on the indicated downstream mediators of Wnt/β-catenin signaling and EMT.

Fig. 6

A–D SMMC-7721 and HepG2 cells were transfected with ZIC1 siRNAs or ZIC1-overexpressing plasmid (OE-ZIC1). A, B Analysis of ZIC1 expression via RT-qPCR. C, D Analysis of ZIC1 expression via western blot. Vector: the empty pcDNA3.1(+) plasmid. E, F β-catenin, cyclin D1, c-myc, E-cadherin, N-cadherin, Vimentin, and MMP2 mRNA expression were detected via RT-qPCR after transfection with si-ZIC1. G, H β-catenin, cyclin D1, c-myc, E-cadherin, N-cadherin, Vimentin, and MMP2 mRNA expression were detected via RT-qPCR after transfection with OE-ZIC1. Data were presented as mean ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.