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. 2021 Dec 20;13(1):12. doi: 10.1038/s41419-021-04464-3

Fig. 8. CircMTO1/miR-541-5p/ZIC1 axis inhibited HCC tumor growth in vivo.

Fig. 8

A Nude mice were subcutaneously injected with (a.) PBS, (b.) cholesterol-conjugated negative control (NC), (c.) cholesterol-conjugated si-circMTO1, (d.) cholesterol-conjugated inhibitor (e.) cholesterol-conjugated si-circMTO1 + cholesterol-conjugated inhibitor. After five injections, tumors were dissected and imaged. B Before injection, the tumor volume was measured and the tumor growth curve plotted. C Tumor weight was calculated on the day the mice were killed. Data represents mean ± SD (n = 5 per group). D–F Expression levels of circMTO1, miR-541-5p, and ZIC1 in xenograft tumors were measured using RT-qPCR. G H&E staining revealed the structure of xenograft tumors. Scale bar; 40 μm. H Changes in ZIC1, β-catenin, c-myc, cyclin D1, E-cadherin, N-cadherin, Vimentin, and MMP2 expression in xenograft tumors were detected through IHC staining. Scale bar; 20 μm. *P < 0.05; **P < 0.01; ***P < 0.001.