Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 20;11:24212. doi: 10.1038/s41598-021-03626-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Visualization of data from individual recordings. (a–f) A representative recording of GCaMP7b-expressing dopamine axons in dorsomedial striatum during an instrumental behavioral task in which animals nosepoke for reward is used to illustrate data visualization and analysis options. a) Peri-stimulus/event time histogram (PSTH) for rewarded nosepokes, in which the mean z-score (black) is plotted along with all individual trials (gray). (b) Mean PSTH for rewarded nosepokes plotting mean z-score with standard error. (c) A multi-event PSTH plot comparing average responses to rewarded (black) vs unrewarded (red) nosepokes. (d) Heatmap of all individual trials for rewarded nosepokes. Trials are shown in chronologically order from top to down. The color scheme for heatmaps in GuPPy is easily modifiable by the user. (e) Example traces illustrating peak detection. Orange dots indicate the detected peaks (left) and an average peak amplitude for all rewarded nosepoke trials is calculated (right). (f) An example trace illustrating area under curve (AUC) calculations. The AUC counted is colored in purple (left) and the average AUC for all rewarded nosepoke trials is calculated (right). (g,h) A representative recording of dLight1.3b in nucleus accumbens during a Pavlovian conditioning task is used to illustrate additional data visualization options comparing recordings across days. (g) PSTHs for days 1, 5, and 10 of Pavlovian training are show separately. A cue (yellow box) predicted reward (dotted line). (h) A heatmap is used to display average PSTHs across days 1–10 in a compact format, allowing easy visualization of the emergence of cue-evoked dopamine with training.